C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
167/40, 167/44
C12Q 1/00 (2006.01) G01N 33/543 (2006.01) G01N 33/545 (2006.01)
Patent
CA 1123336
ABSTRACT OF THE DISCLOSURE The invention relates a method for the demonstration and determination of an antigen or antibody in a test fluid, and to a test kit for the application of this method. More particularly, this invention relates to a method for the detection and determination of an antigen or antibody and a liquid sample containing said antigen or antibody which comprises carrying out the following sequence of process steps:- (A) (a) providing a predetermined quantity of a labelled antibody against said antigen; (b) providing a predetermined quantity of a labelled antibody against said antigen, said labelled antibody being derived from a different animal species than said first antibody; (c) providing an insolubilized antibody against said first antibody; (d) reacting the components of steps (a), (b), and (c) with a liquid sample suspected of containing the antigen to form a solid and a liquid phase; and (e) estimating the amount of labelled antibody in either the liquid or solid phase, said amount being an indication of the amount of antigen present in the liquid sample analyzed: or (B) (a) providing a predetermined amount of an antigen against which the first antibody is directed; (b) providing a second antibody also directed against said antigen, said second antibody derived from a different species of animal than said first antibody; (c) providing an insolubilized antibody directed against either the first or second antibody; (d) providing a labelled antibody directed against either the first or second antibody, provided that both the insolubilized and labelled antibodies are not directed against the same antibody; (e) reacting the components of steps (a) through (d) with a liquid sample suspected of containing the first antibody, thereby forming a solid and a liquid phase; and (f) estimating the amount of labelled antibody in either the liquid or solid phases, said amount being an indication of the amount of the first anti- body present in the sample to be analyzed; the selection of sequence (A) or (B) being entirely dependent in whether an antigen or antibody is to be determined in a given liquid sample.
244622
Kuypers Leonardus P.c.
Wolters Gerrit
Akzona Incorporated
Fetherstonhaugh & Co.
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