Separation of isoenzymes by ion exchange chromatography

Details Separation of isoenzymes by ion exchange chromatography Separation of isoenzymes by ion exchange chromatography

G - Physics

01

N

150/15, 73/93

G01N 33/48 (2006.01) G01N 31/08 (1980.01)

Patent

CA 1038195

ABSTRACT OF THE DISCLOSURE A simple, rapid anion-exchanging column chromato- graphic technique for determining the presence of proteins of different net electrical charge in human serum and tissue, particularly the creatine kinase (CK) and lactate dehydrogenase (LD) isoenzymes in human serum. Samples of serum, layered on mini columns of an ion exchange agent, are eluted stepwise with a buffer solution, preferably Tris-buffered sodium chloride. Elution of the samples is without previous dialysis or other ionization treatment. Column effluents are assayed by spectrophotometric analysis with the Rosalki CK method and/or the Wacker LDH method. Evaluation of sera from patients utilizing these methods reveals isoenzyme patterns which identify a myocardial infarction and other disease associated with an elevated isoenzyme pattern.

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