Colorimetric assay for urea

Details Colorimetric assay for urea Colorimetric assay for urea

G - Physics

01

N

150/14

G01N 31/22 (2006.01) G01N 33/62 (2006.01)

Patent

CA 1044583

ABSTRACT A process for assaying the urea content of fluids such as blood serum, plasma, urine, and spinal fluid using as reagents ortho-phthalaldehyde and N-(1-naphthyl) ethylene- diamine dihydrochloride. The reaction is very specific for urea, and demonstrates a linear relationship of absorbance and concentration for concentrations of urea nitrogen to 250 mg. per deciliter. In addition, both reagents are stable and the reaction does not require a heating bath.

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