G - Physics – 01 – N
Patent
G - Physics
01
N
73/124, 73/55, 7
G01N 33/72 (2006.01) G01N 30/60 (2006.01) G01N 33/66 (2006.01) G01N 30/02 (2006.01) G01N 30/38 (2006.01)
Patent
CA 1138219
ABSTRACT OF THE DISCLOSURE A combination of a microcolumn packed with a suitable absorbent material and a centrifuge tube, is disclosed. A predetermined volume of an eluent being capable of selectively eluting a desired substance through the microcolumn is contained above the packing of the absorbent material. The microcolumn and the centrifuge tube are dimensioned in such a manner that upon centrifugation of the predetermined volume of the eluent through the microcolumn, a level of the eluent remaining in the microcolumn, and a level of the eluent in the centrifuge tube coincide substantially at or above a top surface of the packing of the absorbent material. Consequently, centrifugation of the assembly of the microcolumn and the centrifuge tube causes passage of the predetermined volume of eluent through the microcolumn. Determination or assay of the selectively eluted desired substance may be performed by spectrophotometric methods. The disclosed device and method is particularly adapted for the determination or assay of a relative concentration of glyco- sylated hemoglobin species present in the red blood cells of a specific person. -35-
360734
Graas Joseph E.
Hirons & Rogers
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