Differential assay of creatine phosphokinase isoenzymes

C - Chemistry – Metallurgy – 12 – N

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150/15.5

C12N 1/00 (2006.01) C12Q 1/50 (2006.01)

Patent

CA 1060765

Abstract of the Disclosure A process for the determination of creatine phosphokinase MB-isoenzyme (CPK-MB) level in serum in which CPK is determined before and after MB-activation with 1,4-dimercapto-2,3-dihydroxy butane or 2-mercaptoethanol, preferably in an assay system using the ATP/ADP conversion. The difference in the two values found is due to the CPK-MB present. The invention is of particular utility in the diagnosis and treatment of myocardial damage.

261168

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