Signalling the start of a chemical reaction by tagging...

Details Signalling the start of a chemical reaction by tagging... Signalling the start of a chemical reaction by tagging...

G - Physics

01

N

167/43

G01N 33/53 (2006.01) G01N 21/64 (2006.01) G01N 31/00 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1082594

Abstract of the Disclosure A system for chemically analyzing antigens or antibodies by initiating an antigen-antibody reaction in a reaction zone and measuring the light scattered by a precipitate produced by the re- action to determine the antigen or antibody concentration. In or- der to signal the start of the reaction, a fluorescent tagging sub- stance is included with at least the last antigen or antibody reac- tion component introduced into the reaction zone. The fluorescent tagging substance does not enter into the antigen-antibody reaction and is selected to emit light in a bandwidth spectrally separated from the light scattered by the precipitate. The reaction zone is monitored for the presence of the tagging substance, and when the substance is detected a trigger signal is generated to signal the start of the reaction. By including a tagging substance with each component of the reaction and by monitoring the reaction zone for the presence of each tagging substance, a trigger signal is gener- ated only if each reaction component is present.

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