C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/60 (2006.01) A01H 5/00 (2006.01) C07H 21/00 (2006.01) C12N 5/10 (2006.01) C12N 9/88 (2006.01) C12N 15/10 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2267074
Genes which encode ACC synthase are identified for the geranium and rose plants, specifically Pelargonium X hortorum cv sincerity and Rosa (cardinal red). These genes are shown as modified to achieve a transgenic plant which resists wilting and the like as a result of reduced ethylene production. This alteration is reproduced by the transformed plant. Isolation of high quality mRNA is achieved through the use and adaptation of a 2-butoxyethanol precipitation technique using a large amount of initial tissue in order to achieve critical mass for precipitation.
On a identifié les gènes codant la 1-aminocyclopropane-1-carboxylate (ACC) synthase, dans des plantes telles que le géranium et la rose, notamment Pelargonium x hortorum (variété cultivée "sincérité") et Rosa (rouge cardinal). On a modifié ces gènes pour obtenir une plante transgénique qui résiste au flétrissement et analogue, par suite d'une production réduite d'éthylène, cette modification étant reproduite par la plante transformée. On isole un ARNm de grande qualité en utilisant et adaptant une technique de précipitation de 2-butyxoéthanol, à l'aide d'une grande quantité de tissu initial, afin d'obtenir une masse critique destinée à cette précipitation.
Colorado State University Through Its Agent Colorado State Unive
Sim & Mcburney
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