11-arachidonate-lipoxygenase mutants

Details 11-arachidonate-lipoxygenase mutants 11-arachidonate-lipoxygenase mutants

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) C07C 59/42 (2006.01) C07C 69/732 (2006.01) C07C 409/04 (2006.01) C12N 9/02 (2006.01) C12N 15/82 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2378526

The invention relates to a method for producing a plant lipoxygenase with modified positional specificity toward arachidonic acid and to its utilization for hydroperoxydation of arachidonic acid. The inventive LOX makes it possible to produce for the first time (11S,14Z,12E,8Z,5Z)-11-hydroperoxy-14,12,8,5- eicosatetraenic acids at a large scale. To this end, arachidonic acid is incubated as substrate with the inventive LOX under appropriate conditions. Hydroperoxydation of the arachidonic acid is then effected, preferably in position 11, with secondary products which are hydroperoxylated in position 8 or position 5 or position 11 and 8 and 5.

L'invention concerne un procédé pour la production d'une lipoxygénase végétale à spécificité de position modifiée par rapport à l'acide arachidonique et l'utilisation de cette lipoxygénase pour l'hydroperoxylation de l'acide arachidonique. La lipoxygénase selon l'invention permet en particulier de produire pour la première fois de l'acide (11S,14Z,12E,8Z,5Z)-11-hydroperoxy-14,12,8,5-eicosatétraénoïque en grande quantité. A cet effet, de l'acide arachidonique est incubé comme substrat avec la lipoxygénase selon l'invention, dans des conditions appropriées. L'hydroperoxylation de l'acide arachidonique se produit alors, de préférence en position 11 avec des sous-produits qui sont hydroperoxylés en position 8, en position 5 ou en position 11, 8 et 5.

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