2,5-dkg permeases

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/55 (2006.01) C07K 14/24 (2006.01) C07K 14/26 (2006.01) C12N 15/31 (2006.01) C12P 7/60 (2006.01)

Patent

CA 2417970

The invention provides isolated nucleic acid molecules encoding polypeptides having 2,5-DKG permease activity, and oligonucleotides therefrom. The isolated nucleic acid molecules can be expressed in appropriate bacterial cells to enhance the production of 2-KLG, which can subsequently be converted to ascorbic acid. Further provided are isolated polypeptides having 2,5-DKG permease acitivity, immunogenic peptides therefrom, and antibodies specific therefor. The invention also provides methods of identifying novel 2,5-DKG permeases.

L'invention concerne des molécules d'acide nucléique isolées codant pour des polypeptides présentant une activité de perméase 2,5-DKG, ainsi que des oligonucléotides de celles-ci. Ces molécules d'acide nucléique isolées peuvent être exprimée dans des cellules bactériennes adéquates pour augmenter la production de 2-KLG qui peut ensuite être transformé en acide ascorbique. L'invention concerne également des polypeptides isolés présentant une activité de perméase 2,5-DKG, des peptides immunogènes de ceux-ci, et des anticorps spécifiques à ceux-ci. L'invention concerne également des procédés d'identification de nouvelles perméases 2,5-DKG.

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