C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2664229
The present invention provides reaction mixtures that include blocked oligonucleotides comprising 2'-terminator nucleotides. The blocked oligonucleotides are rendered extendible when the 2'-terminator nucleotides are removed from the oligonucleotides, e.g., via pyrophosphorolysis. The reaction mixtures can be used in various nucleic acid polymerization and/or amplification assays, among many other applications. In addition to reaction mixtures, the invention also provides related methods and reaction mixtures.
La présente invention concerne des mélanges de réaction qui comprennent des oligonucléotides bloqués comprenant des nucléotides terminateurs en 2'. Les oligonucléotides bloqués redeviennent extensibles lorsque les nucléotides terminateurs en 2' sont enlevés des oligonucléotides, par exemple via une pyrophosphorolyse. Les mélanges de réaction peuvent être utilisés dans différents essais de polymérisation et/ou amplification d'acides nucléiques, entre autres nombreuses applications. En plus des mélanges de réaction, l'invention concerne également des procédés correspondants et des mélanges de réaction correspondants.
Bauer Keith A.
Bodepudi Veeraiah
Gelfand David Harrow
Gupta Amar P.
Niemiec John
Borden Ladner Gervais Llp
F. Hoffman-La Roche Ag
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