3,3',5,5'-tetraalkylbenzidines and their use as indicators...

C - Chemistry – Metallurgy – 12 – Q

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150/3, 260/597.7

C12Q 1/26 (2006.01) C12Q 1/28 (2006.01) G01N 31/22 (2006.01)

Patent

CA 1060906

ABSTRACT OF THE DISCLOSURE A diagnostic agent for the determination of hydrogen peroxide or of substances which react with the formation of hydrogenperoxide or of peroxidase or of peroxidate-active substances, employs the reaction of hydrogen peroxide with a chromogen, which reaction is catalyzed by peroxidase or a peroxidate-active substancas, and evaluation of the coloration producedby the reaction, the diagnostic agent employs, as chromogen, a compound of formula (I):- Image in which R1, R2, R3, and R4, which can be the same or different, are straight-chained or branched alkly radicals containing 1 to 6 carbon atoms; the chromogen employed gives more stable coloured radicals in the test than o-tolidine.

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