3'-exonuclease, its preparation and use

Details 3'-exonuclease, its preparation and use 3'-exonuclease, its preparation and use

C - Chemistry, Metallurgy

12

N

C12N 9/22 (2006.01) A61K 38/43 (2006.01) A61K 39/395 (2006.01) C07K 1/22 (2006.01) C07K 16/40 (2006.01) C12N 9/16 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2334046

The present invention concerns a poly(A)-specific 3'-exonuclease activity obtained by chromatographic purification of a crude extract of animal or human cells and to its use in deadenylation of 3'-poly(A) tails of nucleic acids as medicaments, diagnostic reagents or for identifying functional interactors.

L'invention concerne une activité de la 3'-exonucléase poly(A)-spécifique, obtenue par purification chromatographique d'un extrait brut de cellules animales ou humaines, ainsi que son utilisation pour désadényler des 3'-poly(A)-queues d'acides nucléiques, comme médicaments, agents diagnostiques ou pour identifier des agents d'interaction fonctionnels.

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