3' genomic promoter region and polymerase gene mutations...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/40 (2006.01) A61K 39/12 (2006.01) A61K 39/155 (2006.01) A61K 39/165 (2006.01) C07K 14/115 (2006.01) C07K 14/12 (2006.01) C07K 14/135 (2006.01) C12N 7/00 (2006.01) C12N 15/45 (2006.01) C12N 15/47 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2265554

Isolated, recombinantly-generated, attenuated, nonsegmented, negative-sense, single stranded RNA viruses of the Order Mononegavirales having at least one attenuating mutation in the 3' genomic promoter region and having at least one attenuating mutation in the RNA polymerase gene are described. Vaccines are formulated comprising such viruses and a physiologically acceptable carrier. The vaccines are used for immunizing an individual to induce protection against a nonsegmented, negative-sense, single stranded RNA virus of the Order Mononegavirales.

Cette invention concerne des virus d'ARN de l'ordre des Mononegavirales qui sont isolés, produits par recombinaison, atténués, non segmentés, à sens négatif et monocaténaires, et qui présentent au moins une mutation d'atténuation dans la région promotrice 3' du génome et au moins une mutation d'atténuation dans le gène polymérase de l'ARN. On formule des vaccins avec ces virus et un support physiologiquement acceptable. Ces vaccins permettent d'immuniser un individu par induction d'une protection contre un virus monocaténaire, non segmenté et à sens négatif de l'ordre des Mononegavirales.

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