4-(4-hydroxystyryl)pyridine-containing substrates for an...

Details 4-(4-hydroxystyryl)pyridine-containing substrates for an... 4-(4-hydroxystyryl)pyridine-containing substrates for an...

C - Chemistry, Metallurgy

12

Q

C12Q 1/28 (2006.01) C07D 213/06 (2006.01) C07D 213/30 (2006.01) C07D 495/04 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2368190

4-(4-hydroxystyryl) pyridine-containing compounds are disclosed as substrates for use in assays. Also, a method for detecting or quantitating an analyte is provided which includes reacting an enzyme with a 4-(4-hydroxystyryl) pyridine- containing compound to form a reactive intermediate wherein the reactive intermediate deposits covalently on a surface by binding to a receptor, wherein deposited reactive intermediates either directly or indirectly through a label generate a signal which can be detected or quantitated.

L'invention concerne des composés contenant 4-(4-hydroxystyryl) pyridine utilisés comme substrats dans des dosages. L'invention concerne aussi un procédé pour détecter ou quantifier un analyte, qui consiste à faire réagir une enzyme avec un composé contenant 4-(4-hydroxystyryl) pyridine pour former un intermédiaire réactif, l'intermédiaire réactif se déposant par covalence sur une surface au moyen de la liaison à un récepteur, les intermédiaires réactifs déposés générant directement ou indirectement par le biais d'une étiquette un signal qui peut être détecté ou quantifié.

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