C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) A61K 31/70 (2006.01) C07H 19/10 (2006.01) C07H 19/20 (2006.01) C07H 21/00 (2006.01)
Patent
CA 2327286
The invention relates to 5'-modified nucleotides and nucleic acids containing said nucleotides. The invention also relates to methods for integrating 5'- modified nucleotides in nucleic acids and subsequent localized splitting of the nucleic acids on the 5'-modified monomer structural elements. The inventive methods can be used in nucleic acid sequencing, in the creation of nucleic acid libraries, in the detection of mutations, in the production of carrier-bound nucleic acids and for pharmaceutical purposes.
L'invention concerne des nucléotides 5'-modifiées et des acides nucléiques les contenant. L'invention concerne en outre des procédés pour intégrer les nucléotides 5'-modifiés dans des acides nucléiques, ainsi que la dissociation localisée subséquente des acides nucléiques sur les blocs de base de monomères 5'-modifiés. Ces procédés s'utilisent dans le séquençage d'acide nucléique, la création de bibliothèques d'acide nucléique, la mise en évidence de mutations, la préparation d'acides nucléiques liés à des supports, ainsi qu'à des fins pharmaceutiques.
Ansorge Wilhelm
Faulstich Konrad
Europaisches Laboratorium Fur Molekularbiologie (embl)
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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