5' nucleases derived from thermostable dna polymerase

Details 5' nucleases derived from thermostable dna polymerase 5' nucleases derived from thermostable dna polymerase

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) C07H 21/00 (2006.01) C07K 14/47 (2006.01) C12N 1/21 (2006.01) C12N 9/12 (2006.01) C12N 9/16 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2163015

A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.

Moyen permettant un procédé spécifique du site, le clivage d'une structure de clivage d'acide nucléique. Une enzyme de clivage, ayant une activité de nucléase 5' n'affectant pas l'aptitude à la synthèse de l'acide nucléique, sert de fondement à un nouveau procédé de détection de séquences spécifiques d'acide nucléique.

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