5' nucleases derived from thermostable dna polymerase

Details 5' nucleases derived from thermostable dna polymerase 5' nucleases derived from thermostable dna polymerase

C - Chemistry, Metallurgy

12

N

C12N 9/22 (2006.01) C07K 1/22 (2006.01) C12N 1/21 (2006.01) C12N 9/16 (2006.01) C12N 15/55 (2006.01) C12Q 1/44 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2320666

A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.

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