A bank of stem cells for transplantation

C - Chemistry – Metallurgy – 12 – N

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C12N 5/00 (2006.01) C12N 5/0735 (2010.01) C12N 5/075 (2010.01) C12N 15/873 (2010.01) A61P 37/06 (2006.01) C12Q 1/04 (2006.01) C40B 40/02 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2505598

Methods for producing stem cell banks, preferably human, which optionally may be transgenic, e.g., comprised of homozygous MHC allele cell lines are provided. These cells are produced preferably from parthenogenic, IVF, or same- species or cross-species nuclear transfer embryos or by de-differentiation of somatic cells by cytoplasm transfer. Methods for using these stem cell banks for producing stem and differentiated cells for therapy, especially acute therapies, and for screening for drugs for disease treatment are also provide.

L'invention concerne des procédés de constitution de banques de cellules souches, de préférence humaines, éventuellement transgéniques, comprenant, par exemple des lignées de cellules à allèles du CMH homozygotes. Ces cellules sont produites, de préférence, à partir d'embryons produits par parthénogenèse, fécondation in vitro, ou par transfert nucléaire d'espèces identiques ou croisées ou par dédifférenciation de cellules somatiques réalisée par transfert de cytoplasme. L'invention concerne aussi des procédés d'utilisation des banques de cellules souches afin de produire des cellules souches et différentiées pour des thérapies plus spécialement aiguës, et pour le criblage de médicaments destinés à traiter des maladies.

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