C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 11/02 (2006.01) C12N 5/00 (2006.01)
Patent
CA 2547668
The present invention relates to a three dimensional, malleable cell culture composition and method of forming the same comprising hyaluronic acid, chitosan and a polyelectrolytic complex of hyaluronic acid and chitosan. These three components in combination constitute an initial microenvironment for support of stromal cells, and their undifferentiated mesenchymal cell progeny. The tissue engineering device and method of forming the same comprising hyaluronic acid and chitosan and the use of said device with compositions of pluripotent cells and various formulations of cell culture media for repair of tissues is disclosed.
L'invention concerne une composition de culture cellulaire malléable, tridimensionnelle, et un procédé de formation associé, contenant de l'acide hyaluronique, du chitosane et un complexe polyélectrolytique d'acide hyaluronique et de chitosane. Ces trois composés combinés constituent un micro-environnement initial qui accepte les cellules stromales et leur descendance cellulaire mésenchymateuse indifférenciée. Elle porte aussi sur le dispositif de conception de tissus et le procédé de formation associé contenant de l'acide hyaluronique et du chitosane, ainsi que sur l'utilisation de ce dispositif avec des compositions de cellules pluripotentes et plusieurs formulations de supports de culture cellulaire pour la réparation des tissus.
Borden Ladner Gervais Llp
Tissue Engineering Consultants Inc.
LandOfFree
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