C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01)
Patent
CA 2168934
A Brassica sp. genomic clone containing a gene promoter, which directs expression in the tapetum, was isolated. When the promoter was fused with the GUS gene and introduced into B. napus by Agrobacterium mediated transformation, this promoter controlled GUS expression in the tapetum in transgenic B. napus plants. Expression was observed from bud length 2-3 mm, which corresponds to the uninucleate microspore stage, peaked at 4-5 mm which corresponds to the binucleate microspore stage and declined to no detectable level at the open flower stage. There was no GUS expression in other tissues such as root, stem, leaf, sepal, petal or pistil. This promoter will be useful for the temporal and spatial control of gene expression in plants.
Hong Hai Ping
Robert Laurian S.
Gowling Lafleur Henderson Llp
Her Majesty The Queen In Right Of Canada As Represented By The M
National Research Council Of Canada
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