A brassica sp. polygalacturonase gene promoter

Details A brassica sp. polygalacturonase gene promoter A brassica sp. polygalacturonase gene promoter

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) A01H 5/00 (2006.01) C12N 9/24 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2165934

A Brassica sp. genomic clone containing a polygalacturonase gene promoter was isolated. This promoter directs high levels of transcription in the pollen. When the promoter was fused with the GUS gene and introduced into B. napus by Agrobacterium mediated transformation, this promoter controlled GUS expression in microspores and pollen in transgenic B, napus plants. Expression occurred between the late uninucleate microspore stage and the mature pollen grain stage. GUS activity was also identified in tapetal tissue at the binucleate microspore stage. There was no GUS expression in other tissues such as root, stem, leaf, sepal, petal or pistil. This promoter will be useful for the temporal and spatial control of endogenous gene expression in plants.

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