A butanol dehydrogenase enzyme from the bacterium...

Details A butanol dehydrogenase enzyme from the bacterium... A butanol dehydrogenase enzyme from the bacterium...

C - Chemistry, Metallurgy

12

N

C12N 9/04 (2006.01)

Patent

CA 2718628

From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of Achromobacter xylosoxidans.

Une nouvelle enzyme à activité butanol déshydrogénase a été identifiée à partir d'une souche bactérienne isolée à partir d'un échantillon prélevé dans l'environnement, après enrichissement dans un milieu contenant du 1-butanol comme source de carbone. Cette enzyme peut convertir le butyraldéhyde en 1-butanol, l'isobutyraldéhyde en isobutanol et la 2-butanone en 2-butanol, d'où son utilité dans la biosynthèse du butanol en hôtes microbiens recombinants produisant ces substrats. Le gène codant, appelé sadB, a été isolé à partir de la souche identifiée comme un isolat d'Achromobacter xylosoxidans.

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