A cell-free system for initiation of dna replication

Details A cell-free system for initiation of dna replication A cell-free system for initiation of dna replication

C - Chemistry, Metallurgy

12

N

C12N 5/08 (2006.01) C07K 14/47 (2006.01) C12P 19/34 (2006.01) C12Q 1/25 (2006.01) C12Q 1/48 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2258961

A cell-free system for initiating DNA replication under cell cycle control includes S phase cytosol or a fraction thereof in which are incubated G1 phase nuclei, which are co-incubated with S phase nuclei or a fraction thereof and/or cyclins A and/or E complexed to their cognate cyclin dependent kinase (Cdk2). The system may be used to assay for substances which modulate DNA synthesis or initiation thereof, and which have therapeutic potential in a number of contexts.

Système commandé par le cycle des cellules somatiques afin de déclencher la réplication de l'ADN et comprenant un cytosol en phase S, ou une de ses fractions, dans lequel sont incubés des noyaux en phase G1, conjointement avec soit des noyaux en phase S, ou une de leurs fractions, soit des cyclines A ou E mises en complexe avec leur kinase parente dépendante de cycline (Cdk2). On peut utiliser ce système afin d'effectuer la détermination ou le dosage de substances modulant la synthèse de l'ADN ou son déclenchement et possédant un potentiel thérapeutique dans plusieurs contextes.

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