B - Operations – Transporting – 01 – J
Patent
B - Operations, Transporting
01
J
B01J 20/22 (2006.01) B01D 15/08 (2006.01) B01D 15/36 (2006.01) B01J 20/32 (2006.01) B01J 39/26 (2006.01)
Patent
CA 2349948
The present invention relates to a novel sorbent suitable for use as a stationary phase in a chromatography column, the core of which consists of an organic polymer of synthetic or natural origin. Further, the carrier according to the invention exhibits a plurality of covalently bonded non-aromatic zwitterionic groups on its surface. Additionally, the invention also relates to a method for purifying a particular biological macromolecule, such as a protein or a nucleic acid, by zwitterionic ion exchange chromatography as well as an ion exchange column suitable for use in zwitterionic ion exchange chromatography.
La présente invention concerne un nouveau sorbant pouvant être utilisé comme phase stationnaire dans une colonne de chromatographie, dont la partie centrale est constituée d'un polymère organique d'origine naturelle ou synthétique. En outre, le support selon l'invention comporte en surface une pluralité de groupes zwittérioniques non aromatiques à liaison covalente. L'invention concerne également un procédé de purification d'une macromolécule biologique spécifique, telle qu'une protéine ou un acide nucléique, par chromatographie par échange d'ions zwittérioniques, ainsi qu'une colonne échangeuse d'ions pouvant être utilisée en chromatographie par échange d'ions zwittérioniques.
Irgum Knut
Viklund Camilla
Goudreau Gage Dubuc
Irgum Knut
Merck Patent Gmbh
Viklund Camilla
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