A composition for use in fluorescence assay systems

Details A composition for use in fluorescence assay systems A composition for use in fluorescence assay systems

G - Physics

01

N

G01N 33/58 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2316313

The present invention relates to a composition for use in fluorometry, a method of fluorometry and the use of a class of new reagents in fluorometry. The composition comprises a substrate which is a non-fluorescent derivative of a fluorophore, and a shifting reagent which shifts the absorbance wavelength maximum of the fluorophore which maximum is naturally above 450 nm. The shifting reagent is present in an amount predetermined to shift the maximum to a preset value.

La présente invention concerne une composition destinée à s'utiliser en fluorimétrie, une méthode de fluorimétrie et l'utilisation d'une classe de nouveaux réactifs en fluorimétrie. La composition comprend un support qui est un dérivé non fluorescent d'un fluorophore et un réactif de déplacement qui modifie la longueur d'onde d'absorbance maximale du fluorophore, naturellement supérieure à 450 nm. Le réactif de déplacement est présent en quantité prédéterminée pour modifier la longueur d'onde maximale dans une valeur prédéfinie.

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