A concentrated medium and its usage

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/071 (2010.01) C12N 5/00 (2006.01)

Patent

CA 2721585

A concentrated culture solution used for culturing chinese hamster ovary cells on large scale, which comprises basic medium and high-dose additives. A method of adding the culture solution to CHO cells during fed-batch culturing.

La présente invention concerne une solution de culture qui convient à la culture à grande échelle de cellules ovariennes de hamster de Chine et qui comprend un milieu basique et des doses élevées d'additifs. L'invention concerne également un procédé permettant, pendant une culture à alimentation discontinue, d'apporter cette solution de culture aux cellules ovariennes du hamster de Chine.

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