A conditional replication and expression system

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/86 (2006.01) A61K 48/00 (2006.01) C07K 14/015 (2006.01) C12N 5/10 (2006.01) C12N 15/79 (2006.01) C12N 15/864 (2006.01)

Patent

CA 2278341

The present invention relates to the utilization of conditionally replicating recombinant nucleic acid molecules rescued from the integrated state for the expression of foreign proteins. The usefulness of the system is illustrated with a conditionally replicating recombinant nucleic acid molecule encoding adeno-associated virus (AAV) capsid proteins. The present invention also relates to methods employing said conditionally replicating recombinant nucleic acid molecules for the packaging of recombinant AAV nucleic acid molecules into AAV capsids. The present invention also relates to packaging cell lines for recombinant AAV, expressing both the AAV rep and cap-genes.

L'invention concerne l'utilisation de molécules d'acide nucléique recombinées à réplication conditionnelle, récupérées lorsque ledit acide nucléique est à l'état intégré, pour l'expression de protéines étrangères. L'utilité dudit système est illustrée par une molécule d'acide nucléique recombinée à réplication conditionnelle, codant les capsides d'un virus ayant les caractéristiques d'un adénovirus (AAV). L'invention porte aussi sur des procédés dans lesquels lesdites molécules d'acide nucléique recombinées à réplication conditionnelle sont utilisées pour l'encapsidation de molécules d'acide nucléique AAV recombinées dans des capsides d'AAV. Elle se rapporte encore à des lignées cellulaires d'encapsidation pour le virus recombiné AAV, exprimant les gènes AAV rep et cap.

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