C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 14/705 (2006.01) B03C 1/00 (2006.01) C07K 1/113 (2006.01) C12N 15/10 (2006.01)
Patent
CA 2541241
The present invention relates to a continuous process and a system for macromolecular assembly and capture (refolding or proteins (e.g. refolding of HAT human .beta.2-microglobulin), hybridization of nucleic acid, nucleic acid analogues, and protein-nucleic acid chimera, aggregation of carbohydrates, and assembly of nanostructures/nanomaterials. The process may comprise the steps of providing fluid compositions comprising at least one of said macromolecular substances in a predominantly unassembled form, providing a dispersion comprising coated, essentially non-porous magnetic particles, combining said fluid compositions and the dispersion of magnetic particles thereby providing a continuous stream, passing said continuous stream through a first mixing device thereby forming magnetic complexes each comprising a magnetic particle and a plurality of macromolecular assembly species; passing the continuous stream through a first capture compartment of a magnetic separator thereby capturing said magnetic complexes; and separating said magnetic complexes from said continuous stream, and isolating said macromolecular assembly species.
L'invention concerne un procédé continu et un système pour l'assemblage et la capture macromoléculaire (renaturation de protéines (par exemple, renaturation de la ?2-microglobuline humaine HAT), l'hybridation d'acides nucléiques, d'analogues d'acides nucléiques, et de chimères protéine-acide nucléique, l'agrégation de glucides ainsi que l'assemblage de nanostructures/nanomatériaux. Ce procédé peut consister à: obtenir d'abord des compositions fluides qui contiennent au moins une desdites substances macromoléculaires sous une forme essentiellement non assemblée; obtenir ensuite une dispersion qui contient des particules magnétiques enrobées essentiellement non poreuses; puis, combiner lesdites compositions fluides et la dispersion des particules magnétiques pour former un flux continu; faire passer le flux continu par un premier mélangeur pour obtenir des complexes magnétiques comprenant chacun une particule magnétique et une pluralité d'espèces d'assemblage macromoléculaire; faire passer le flux continu par un premier compartiment de capture d'un séparateur magnétique pour capturer lesdits complexes magnétiques; et enfin, extraire lesdits complexes magnétiques du flux continu et isoler les espèces d'assemblage macromoléculaire.
Buus Soren
Ferre Henrik
Hansen Dennis B.
Hobley Timothy J.
Thomas Owen R. T.
Danmarks Tekniske Universitet
Kobenhavns Universitet
Sim & Mcburney
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