C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 1/20 (2006.01) C07K 7/16 (2006.01) C07K 14/575 (2006.01) C07K 14/585 (2006.01) C07K 14/655 (2006.01)
Patent
CA 2582083
The invention encompasses a process for purifying a peptide comprising loading a peptide onto a RP-HPLC column; washing the column with an aqueous solution of a pharmaceutically acceptable counterion salt; and eluting the peptide from the column with a solvent mixture of a organic solvent and an acid of the pharmaceutically acceptable counterion, wherein the aqueous solution has a pH of at least about 6.
La présente invention concerne un processus de purification de peptide qui consiste à charger un peptide sur une colonne, à laver cette colonne avec une solution aqueuse d'un sel de contre-ion répondant aux normes pharmaceutiques et, à éluer le peptides de la colonne avec un mélange solvant d'un solvant organique et d'un acide de ce contre-ion répondant aux normes pharmaceutiques, cette solution aqueuse possédant un pH d'au moins six.
Alon Hagi
Butilca Gabriel-Marcus
Eidelman Chaim
Elster Shai
Ivchenko Alexander
Heenan Blaikie Llp
Novetide Ltd.
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