A cytochrome p450 enzyme associated with the synthesis of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/29 (2006.01) C07K 14/415 (2006.01) C12N 9/02 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01) C12P 7/64 (2006.01) C12P 17/02 (2006.01)

Patent

CA 2410663

An isolated nucleic acid fragment encoding a cytochrome P450 enzyme associated with the synthesis of plant D12-epoxy fatty acids is disclosed. The invention also relates to making a chimeric construct encoding all or a portion of the cytochrome P450 enzymes associated with the synthesis of plant D12-epoxy fatty acids, in sense or antisense orientation, wherein expression of the chimeric construct results in production of altered levels of the cytochrome P450 enzymes associated with the synthesis of plant D12-epoxy fatty acids in a transformed host cell.

L'invention concerne un fragment isolé d'acide nucléique codant pour une enzyme cytochrome P450 associée à la synthèse des acides gras D12-époxydes dans des végétaux. L'invention concerne aussi la fabrication d'une structure chimère codant la totalité ou une partie des enzymes cytochromes P450, associées à la synthèse des acides gras D12-époxydes dans des plantes, avec orientation sens ou antisens, l'expression de la structure chimère permettant la production de taux modifiés des enzymes cytochromes P450 associées à la synthèse des acides gras D12-époxydes dans des plantes à l'intérieur d'une cellule hôte transformée.

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