C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) C12N 1/19 (2006.01) C12N 9/02 (2006.01) C12N 15/63 (2006.01) C12P 7/40 (2006.01) C12P 7/64 (2006.01) C12Q 1/68 (2006.01) C40B 30/04 (2006.01)
Patent
CA 2523786
The present invention relates to a .DELTA.12 fatty acid desaturase able to catalyze the conversion of oleic acid to linoleic acid (LA; 18:2). Nucleic acid sequences encoding the desaturase, nucleic acid sequences that hybridize thereto, DNA constructs comprising the desaturase gene, and recombinant host microorganisms expressing increased levels of the desaturase are described. Methods of increasing production of specific .omega.-3 and/or .omega.-6 fatty acids are described by overexpression of the .DELTA.12 fatty acid desaturase or by disruption of the native gene.
La présente invention concerne une désaturase d'acide gras ?12 capable de catalyser la conversion de l'acide oléique en acide linoléique (LA; 18:2). L'invention concerne aussi des séquences d'acide nucléique qui codent pour la désaturase, des séquences d'acides nucléiques qui s'hybrident à celle-ci, des séquences d'ADN hybrides comprenant le gène de désaturase, et des micro-organismes hôtes recombinés exprimant des taux supérieurs de désaturase. L'invention a également pour objet des procédés pour augmenter la production d'acides gras ?-3 et/ou ?-6 spécifiques, grâce à la surexpression de la désaturase d'acide gras ?12 ou à la dislocation du gène natif.
Yadav Narendra S.
Zhang Hongxiang
Bennett Jones Llp
E.i. Du Pont de Nemours And Company
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