A device and method for the determination of protein domain...

Details A device and method for the determination of protein domain... A device and method for the determination of protein domain...

C - Chemistry, Metallurgy

12

Q

C12Q 1/37 (2006.01) C07K 1/12 (2006.01) C12M 1/40 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2243230

A method and device to assist in the determination of protein domain boundaries is described. In particular the device is designed to provide a high throughput of proteolytic digestion of proteins to identify domains and their boundaries, for use in protein structure determination, in a manner that is amenable to automation. Proteases are immobilized in a convenient format such as a microtitre plate and preferably arranged in a matrix thereby allowing for simultaneous degradation of a protein by a number of proteases at a number of concentrations.

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