A device for binding a target entity to a bait entity and...

G - Physics – 01 – N

Patent

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G01N 33/543 (2006.01)

Patent

CA 2566662

The present invention pertains to a device for binding a target entity onto a bait entity that is immobilized on said device, comprising : a) a lipid layer having a negative net charge in an aqueous solution at a neutral pH; b) a two- dimensional matrix of anchoring complexes that are bound to said lipid layer, wherein each of said anchoring complexes comprises : (i) a fusion complex comprising an Annexin protein fused to a partner rmolecule; and (ii) a bait entity. It also concerns various uses of said device, including for detection and pharmaceutical purposes.

L'invention porte sur un dispositif liant une entité cible à une entité appât immobilisée sur ledit dispositif lequel comprend: a) une couche lipidique à charge nette négative présente dans une solution aqueuse de pH neutre; une matrice bidimensionnelle de complexes d'ancrage liés à ladite couche lipidique, chacun des complexes d'ancrage comprenant: (i) un complexe fusionné comprenant une protéine d'annexine fusionnée avec une molécule partenaire; (ii) et une entité appât. L'invention porte également sur différentes utilisations dudit dispositif dont la détection et des objectifs à caractère pharmaceutique.

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