A device for detecting bacterial contamination and method of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/00 (2006.01) C12Q 1/04 (2006.01) C12Q 1/24 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 2412071

A device and method for detecting the presence or absence of a prokaryotic microorganism are provided, comprising the steps of identifying a protein, such as a microbial-specific protease that characterizes the presence of a specific prokaryotic microbe and thereby provides a marker for that microbe; detecting the protease that is a marker for the presence of a specific prokaryotic microbe by cleaving a substance when the protease is present; and signaling the presence of that protease when cleavage has occurred. More specifically, the method comprises identifying at least one outer membrane protein or a secreted protein that is unique to a particular microbial pathogen such as for example Listeria monocytogenes and that is substrate specific.

L'invention concerne un dispositif et un procédé de détection de la présence ou de l'absence de micro-organisme procaryote, le procédé consistant à identifier une protéine, telle qu'une protéase microbienne qui caractérise la présence d'un microbe procaryote spécifique et fournit ainsi un marqueur pour ce microbe, à détecter la protéase marqueur révélant la présence d'un microbe procaryote spécifique par clivage d'une substance lorsque la protéase est présente, et à signaler la présence de cette protéase lorsque le clivage est réalisé. Le procédé consiste, plus spécifiquement, à identifier au moins une protéine de membrane extérieure ou une protéine sécrétée, unique d'un pathogène microbien particulier tel que, par exemple, Listeria monocytogenes et qui présente une spécificité de substrat.

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