A digital imaging system for assays in well plates, gels and...

G - Physics – 01 – N

Patent

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G01N 21/84 (2006.01) C40B 30/10 (2006.01) C40B 60/12 (2006.01) G01N 33/483 (2006.01) G06T 7/00 (2006.01)

Patent

CA 2299325

A method and apparatus are disclosed for use in an area digital imaging system for assays to extract targets on a specimen containing an array of targets that may not be arranged in perfect regularity. A matrix is defined of nominal target locations including a probe template of predefined, two-dimensional size and shape at each of a plurality of fixed, predefined grid points on the specimen, and a determination is made of the most probable location of the probe template corresponding to a specific target by sensing both pixel intensity and the spatial distribution of pixel intensities in an image of the specimen at a plurality of locations in the vicinity of a nominal target location.

L'invention concerne un procédé et un dispositif, utiles dans un système d'imagerie numérique destiné à des dosages servant à extraire des cibles d'un spécimen contenant un groupe de cibles lesquelles peuvent n'être pas disposées de manière parfaitement régulière. On délimite une matrice d'emplacements de cibles nominales comprenant une matrice de sondes de sonde de forme et dimension bidimensionnelles prédéfinies au niveau de chaque point d'une pluralité de points d'une grille, fixes, déterminés et situés sur le spécimen, et on détermine l'emplacement le plus probable de la matrice correspondant à une cible spécifique en détectant à la fois l'intensité des pixels et la répartition spatiale des intensités des pixels dans une image du spécimen, au niveau de plusieurs emplacements situés au voisinage d'un emplacement cible nominal.

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