A digital imaging system for assays in well plates, gels and...

Details A digital imaging system for assays in well plates, gels and... A digital imaging system for assays in well plates, gels and...

G - Physics

01

N

G01N 21/84 (2006.01) G01N 21/64 (2006.01) G01N 33/483 (2006.01) G02B 13/22 (2006.01) G02B 21/08 (2006.01)

Patent

CA 2263226

An electronic imaging system is disclosed, for assessing the intensity of colorimetric, fluorescent or luminescent signal in a matrix consisting of wells, microwells, hybridization dot blots on membranes, gels, or other specimens. The system includes a very sensitive area CCD detector (18), a fast, telecentric lens (22) with epi-illumination (44), a reflective/transmissive illumination system, an illumination wavelength selection device (34), and a light-tight chamber (24). A computer and image analysis software are used to control the hardware, correct and calibrate the images, and detect and quantify targets within the images.

L'invention concerne un système électronique d'imagerie permettant d'évaluer l'intensité d'un signal colorimétrique, fluorescent ou luminescent dans une matrice comportant des puits, des micropuits, des taches d'hybridation sur membranes, gels ou autres spécimens. Le système comprend un détecteur (18) à surface très sensible à couplage de charge (CCD), une lentille télécentrique (22) à objectif rapide à illumination verticale (44), un système d'illumination par réflexion/transmission, un dispositif (34) de sélection de longueur d'onde d'illumination et une chambre étanche à la lumière (24). On utilise un ordinateur et un logiciel d'analyse d'images pour gérer le matériel, corriger et étalonner les images, détecter et quantifier des cibles dans lesdites images.

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