A fast method of transforming competent cells

Details A fast method of transforming competent cells A fast method of transforming competent cells

C - Chemistry, Metallurgy

12

N

C12N 15/64 (2006.01) C12N 1/20 (2006.01)

Patent

CA 2580300

The invention relates to a fast method of transforming competent cells, comprising: a. mixing a plasmid DNA with the competent cells suspending within ionic solution to form a mixture; b. plating the mixture on a warm selective medium; and c. culturing the mixture on the medium; wherein the ionic solution comprises divalent cation selected from the group consisting of Ca2+, Mg2+, Mn2+, Zn2+, Cu2+, Cd2+, Fe2+, Sr2+ and Co2+, and provided that the ionic solution does not include Ca2+ alone.

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