A feeder cell-free culture medium and system

Details A feeder cell-free culture medium and system A feeder cell-free culture medium and system

C - Chemistry, Metallurgy

12

N

C12N 5/0735 (2010.01) C12N 5/071 (2010.01) A61K 35/12 (2006.01)

Patent

CA 2697518

A cell culture medium and system are provided which eliminates or at least reduces the need for feeder cells. The cell culture medium comprises one or more factors that are normally secreted and/or produced by a feeder cell and a synthetic chimeric protein comprising IGF-I and a portion of vitronectin. The cell culture medium is particularly suitable for propagating human embryonic stem cells and keratinocytes. This invention also relates to compositions and methods which utilize the cells cultured in the cell culture medium of the invention.

La présente invention concerne un milieu de culture cellulaire et un système qui éliminent ou au moins réduisent le besoin de cellules nourricières. Le milieu de culture cellulaire comprend un ou plusieurs facteurs qui sont généralement secrétés et/ou générés par une cellule nourricière et une protéine chimère synthétique contenant de l'IGF-I et de la vitronectine. Le milieu de culture cellulaire convient particulièrement à la propagation de cellules souches embryonnaires humaines et de kératinocytes. L'invention porte aussi sur des compositions et des procédés qui utilisent les cellules cultivées dans le milieu de culture cellulaire de l'invention.

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