C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12N 15/11 (2006.01)
Patent
CA 2289961
The application provides genes causative of rheumatoid arthritis, which are located within ~ 1 centimorgan from DNA sequences to which the microsatellite markers D1S214, D1S253, D8S556, DXS1001, DXS1047, DXS1205, DXS1227 and/or DXS1232 are hybridized: a method for diagnosing rheumatoid arthritis, comprising amplifying the genomic DNA of a subject by PCR using at least one of the microsatellite markers as primer and comparing the PCR products thereof with the PCR products prepared in the same manner using the genomic DNA of a normal control; and a method for identifying the causative factors of rheumatoid arthritis comprising the same as described above.
L'invention concerne une méthode pour diagnostiquer l'arthrite rhumatoïde, qui se caractérise par l'amplification PCR de l'ADN génomique d'un patient à l'aide d'un gène responsable de l'arthrite rhumatoïde, situé au plus à +/- 1 centimorgan en dehors d'une séquence d'ADN hybridable avec des marqueurs microsatellites D1S214, D1S253, D8S556, DXS1001, DXS1047, DXS1205, DXS1227 et/ou DXS1232 et au moins un des marqueurs microsatellites précités, comme amorce, ainsi que par la comparaison des produits de PCR obtenus avec les produits de PCR correspondants d'un sujet sain.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Shiozawa Shunichi
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