A gene delivery vehicle expressing the apoptosis-inducing...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/861 (2006.01) A61K 48/00 (2006.01) C07K 14/01 (2006.01) C12N 5/10 (2006.01) C12N 7/01 (2006.01) C12N 15/34 (2006.01) C12N 15/86 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2286165

The invention relates to gene delivery vehicles which comprise nucleic acid molecules encoding apoptosis-inducing proteins VP2 and/or apoptin (VP3) like activity. VP2 and VP3 are viral proteins of the Chicken Anaemia Virus. Also, the invention relates to anti-tumor therapies. Infection of various human tumor cells with the gene delivery vehicles of the invention will result in the induction of apoptosis in tumor cells and much reduced apoptosis, if at all, in normal diploid, non- transformed/non-malignant cells. Also the invention relates to the diagnosis of cancer, and related forms of hyperplasia, metaplasia and dysplasia.

L'invention concerne des véhicules d'apport génique qui comprennent des molécules d'acide nucléique codant pour les protéines VP2 induisant l'apoptose et/ou pour une activité du type apoptine (VP3). VP2 et VP3 sont des protéines virales du virus de l'anémie infectieuse du poulet. De même, l'invention concerne des thérapies antitumorales. L'infection de diverses cellules tumorales humaines au moyen des véhicules d'apport génique de l'invention a pour effet d'induire l'apoptose dans des cellules tumorales ainsi qu'une apoptose bien moindre, si apoptose il y a, dans des cellules normales, diploïdes, non transformées/non malignes. L'invention concerne encore le diagnostic du cancer et des formes associées d'hyperplasie, métaplasie et dysplasie.

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