A gene encoding a putative efflux protein for...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/31 (2006.01) C07K 14/21 (2006.01) C12N 1/21 (2006.01) C12P 7/42 (2006.01)

Patent

CA 2304830

This invention relates to the isolation of a novel putative efflux gene from Pseudomonas mendocina. The putative efflux gene is useful for probing an organism's efflux system to gain an understanding of the mechanisms of solvent tolerance. The invention further provides a Pseudomonas mendocina strain deficient in this gene. This strain is unable to grow in the presence of chloramphenicol and, compared to the wildtype strain, grows slowly in the presence of high concentrations of PHBA.

Cette invention concerne l'isolement d'un nouveau gène d'efflux présumé présent dans Pseudomonas mendocina. Ce nouveau gène d'efflux présumé est utile pour explorer un système d'efflux de l'organisme afin de mieux comprendre les mécanismes de tolérance aux solvants. Cette invention concerne également une souche de Pseudomonas mendocina dans laquelle ce gène est déficient. Cette souche est incapable de se développer en présence de chloramphénicol et, comparée à la souche de type sauvage, croît lentement en présence de concentrations élevées d'acide p-hydroxybenzoïque (PHBA).

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