A gene expression system utilizing an rna polymerase...

Details A gene expression system utilizing an rna polymerase... A gene expression system utilizing an rna polymerase...

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) C07K 14/61 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12N 15/10 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2162867

The present invention relates to a gene expression system in eukaryotic cells. In particular, it relates to a self-initiating and self- sustaining gene expression system utilizing an RNA polymerase prebound to a DNA construct before the introduction of the complex into cells. This system is capable of functioning in the cell cytoplasm without integration into the genome of host cells, and does not require host cell factors for initiation and maintenance of gene expression. Therefore, the invention has a wide range of applications in in vitro and in vivo gene expression, including gene therapy in resting cells.

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