C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/70 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12Q 1/04 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01)
Patent
CA 2137809
A diagnostic assay for detecting the presence of an infectious herpes virus in a specimen and a genetically engineered cell line for use in such assay are disclosed. The cell line used in the assay expresses a reporter gene only if infectious herpes virus is present in the specimen. The assay involves inoculating a DNA-transfected cell line with a specimen suspected of containing a herpes virus, allowing a sufficient period of time for the herpes virus infectious cycle to proceed, and detecting and quantifying the number of herpes virus-infected cells to determine the number of infectious herpes virus virions in the specimen. The cell line is a DNA-transfected cell line susceptible to infection by a herpes virus which is stably transformed with a chimeric gene compris- ing a herpes virus inducible promoter and a gene coding for an enzyme, the expression of the enzyme being dependent upon and quantitatively proportional to the presence of herpes virus. A kit for such assay is also provided.
Battison Williams Dupuis
Washington University
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