A group of .alpha.-amylases and a method for identification...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/56 (2006.01) C07H 21/00 (2006.01) C07K 19/00 (2006.01) C11D 3/26 (2006.01) C11D 3/33 (2006.01) C12N 1/20 (2006.01) C12N 9/28 (2006.01) C12N 15/10 (2006.01) C12N 15/62 (2006.01) C12N 15/70 (2006.01) C12N 15/76 (2006.01) C12Q 1/40 (2006.01) C12Q 1/68 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2452029

The invention relates to a novel group of .alpha.-amylases, belonging to a common sequence region and proteins sufficiently similar to said .alpha.- amylases with amylolytic function, methods for production thereof and diverse possible applications for said amylolytic proteins, in particular in washing and cleaning agents. The invention further relates to a PCR-based method for identification and production of novel .alpha.-amylases from isolated genomic DNA, in particular from DNA isolated from collections of micro-organisms and particular primer oligonucleotides and application thereof in said method.

L'invention concerne un nouveau groupe d'.alpha.-amylases appartenant à un espace de séquence commun, ainsi que des protéines à fonction amylolytique suffisamment analogues à ces .alpha.-amylases, un procédé d'obtention de ces composés et diverses possibilités d'utilisation de ces protéines amylolytiques, en particulier dans des agents de lavage et des détergents. L'invention concerne également un procédé à base PCR, permettant l'identification et l'obtention de nouvelles .alpha.-amylases à partir d'ADN génomique isolé, en particulier à partir d'ADN isolé à partir de collections de micro-organismes, ainsi que des oligonucléotides d'amorçage et leur utilisation dans un tel procédé.

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