A group of nucleic acid fragments for prevention of hiv...

C - Chemistry – Metallurgy – 07 – H

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C07H 21/00 (2006.01) A61K 48/00 (2006.01) A61P 31/18 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12N 15/88 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2509827

The invention provides a group of nucleic acid fragments, shown in the sequence listing, for prevention of HIV infection or AIDS and the usage thereof. In the invention, a series of RNA fragments, which are highly homogenous to all the published HIV gene sequences, were obtained by homology compare. The double-stranded RNA (dsRNA) derived from these fragments can effectively inhibit the expression of the HIV genes. The RNA transcribed by plasmid, also can suppress the expression of the HIV in the cell. After the adenovirus or associated virus which carry DNA corresponding above RNA infect the cell, the transcription dsRNA can inhibit the expression of the HIV genes.

L'invention concerne un groupe de fragments d'acide nucléique, mentionnés dans la liste de séquences, s'utilisant pour prévenir l'infection par VIH ou le SIDA, ainsi que leur utilisation. Selon l'invention, une série de fragments d'ARN, qui présentent un pourcentage élevé d'homologie avec toutes les séquences de gènes VIH publiées, ont été obtenus par comparaison d'homologie. L'ARN à brin double (ARN¿db?) dérivé de ces fragments peut inhiber de manière efficace l'expression des gènes du VIH. L'ARN transcrit par plasmide peut également supprimer l'expression du VIH dans la cellule. Une fois la cellule infectée par l'adénovirus ou par le virus associé portant l'ADN correspondant à l'ARN mentionné ci-dessus, la transcription de l'ARN¿db? peut inhiber l'expression des gènes du VIH.

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