C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/00 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2446207
The present invention provides a method for screening of a large number of compounds for their ability to modulate the expression of genes. The method uses gene trap technology and comprises the steps of transfecting a population of cells with a gene-trap vector, sorting cells according to their level of fluorescence, distributing sorted cells into pools and expanding the pools to obtain a sufficient number of cells representing each trapped gene to permit distinction of the effect of a test compound over controls, exposing the cells to the test compounds and identifying compounds which alter the fluorescence distribution pattern of cells using FACS analysis.
La présente invention concerne un procédé de criblage d'un grand nombre de composés en vue de déterminer leur capacité de moduler l'expression génétique. Le procédé utilise la technologie de piégeage de gènes et comporte les étapes de transfection d'une population de cellules au moyen d'un vecteur de piégeage de gènes, le tri des cellules selon leur niveau de fluorescence, la distribution des cellules triées en groupes et l'expansion des groupes en vue d'obtenir un nombre suffisant de cellules représentant chaque gène piégé permettant de distinguer l'effet d'un composé de test sur les témoins, l'exposition des cellules aux composés de test et l'identification des composés qui modifient la configuration de la distribution de la fluorescence des cellules au moyen d'une analyse de trieur de cellules à fluorescence.
Hangauer David G.
Mielnicki Lawrence Mark
Pruitt Steven C.
Stewart Carleton C.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Health Research Inc.
The Research Foundation Of State University Of New York
LandOfFree
A high throughput assay for identification of gene... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with A high throughput assay for identification of gene..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and A high throughput assay for identification of gene... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-2069507