A high throughput assay using fusion proteins

Details A high throughput assay using fusion proteins A high throughput assay using fusion proteins

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 1/22 (2006.01) C07K 19/00 (2006.01) C12N 9/90 (2006.01) G01N 33/543 (2006.01) G01N 33/60 (2006.01) G01N 33/68 (2006.01) G01N 33/94 (2006.01)

Patent

CA 2231330

This application describes a high throughput assay for screening for compounds capable of binding to a fusion protein which consists of a target protein and an FK506-binding protein. The method for preparing the DNA encoding for the fusion protein and for expressing that DNA is also described in the application. The invention also discloses the recombinant DNA and protein sequences for several fusion proteins.

L'invention porte sur un dosage à rendement élevé utilisé pour sélectionner des composés capables de se lier à une protéine hybride composée d'une protéine-cible et d'une protéine fixant FK506. L'invention concerne également le procédé de préparation de l'ADN codant la protéine hybride, et d'expression de cet ADN. L'invention se rapporte également aux séquences protéiques et d'ADN de recombinaison pour plusieurs protéines hybrides.

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