G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/483 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2429567
A method for identifying a Na+ channel blocker, including providing a cell containing a Na+ channel blocker, demonstrating both a transient and a persistent current. The cell includes a potassium (K) channel and a Na/K AtPase (Na+ pump). A florescent dye is disposed into the well. The florescent dye is sensitive to change in cell membrane potential in order to enable optical measurement of cell membrane potential. A Na+ channel blocker, to be identified, is added to the well and a stimulating current is passed through the cell in an amount sufficient to generate an action potential before and after the addition of the Na+ channel blocker. Thereafter, a change in cell membrane potential is opticaly measured.
Procédé pour identifier un bloqueur de canal Na+, qui consiste à fournir une cellule contenant un bloqueur de canal Na+ manifestant un courant transitoire et un courant persistant. La cellule comprend un canal de potassium (K) et une Na/K AtPase (une pompe Na+). Un colorant fluorescent est déposé dans le puits. Le colorant fluorescent est sensible au changement dans le potentiel de la membrane pour permettre la mesure optique du potentiel de la membrane cellulaire. Pour pouvoir être identifié, un bloqueur de canal Na+ est ajouté au puits et un courant de stimulation passe à travers la cellule avec une intensité suffisante pour générer un potentiel d'action avant et après l'ajout du bloqueur de canal Na+. Cela permet de mesurer optiquement un changement de potentiel de la membrane cellulaire.
Adorante Joseph S.
Donello John E.
Ehring George R.
Allergan Inc.
Gowling Lafleur Henderson Llp
LandOfFree
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