A highly sensitive method for detecting environmental insults

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C12N 9/02 (2006.01) C12N 15/53 (2006.01) C12N 15/70 (2006.01) C12Q 1/02 (2006.01) C12Q 1/66 (2006.01)

Patent

CA 2150232

Subtle changes in environmental stress can now be detected and measured at sublethal levels as a generic response to environmental stress. The present invention provides a method to detect changes in the environmental stress level. The stress change is indicated as a change in the liminescence output of a genetically engine microorganism. In the present invention, the luminescence gene complex is under the control of a stress inducible promoter.

Des variations subtiles du stress environnemental peuvent à présent être détectées et mesurées à des niveaux inférieurs au niveau létal sous forme d'une réponse générique à ce stress. La présente invention se rapporte à un procédé permettant de détecter des variations du niveau de stress environnemental. La variation du stress est indiquée sous forme d'une variation de la luminescence générée par un micro-organisme obtenu par technique génétique. Selon la présente invention, le complexe du gène de luminescence est soumis au contrôle d'un promoteur inductible par le stress.

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