A homogeneous time resolved fluorescence based test system

G - Physics – 01 – N

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G01N 33/542 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2646093

The present invention concerns a fluorescence resonance energy transfer based high throughput test system to measure the formation of the HIV gp41 six- helix bundle. In a first embodiment the current invention relates to a homogeneous time resolved fluorescence-based test system comprising a first helical polypeptide consisting essentially of the sequence of IQN36 (SEQ ID NO: 1); a second helical polypeptide consisting essentially of the sequence of C34 (SEQ ID NO: 2) wherein said IQN36 is labeled with a light emitting fluorophore and said C34 is labeled with an ultra-violet excitable fluorophore.

La présente invention concerne un système de test de capacité élevée basé sur le transfert d'énergie de résonance de fluorescence pour mesurer la formation du faisceau à 6 hélices gp41 du VIH. Dans un premier mode de réalisation, la présente invention concerne un système de test basé sur la fluorescence en temps résolu en milieu homogène comprenant un premier polypeptide hélicoïdal constitué essentiellement de la séquence du peptide IQN36 (SEQ ID n° : 1) ; un second polypeptide hélicoïdal constitué essentiellement de la séquence du peptide C34 (SEQ ID n° : 2), ledit IQN36 étant marqué par un fluorophore émettant de la lumière et ledit C34 étant marqué par un fluorophore excitable par les ultraviolets.

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